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Член начал своё движение быстрее.
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PubMed Cite this publication. In Saccharomyces cerevisiae the activity for the lactate-proton symporter is dependent on JEN1 gene expression. Pichia pastoris was transformed with an integrative plasmid containing the JEN1 gene. After 24 h of methanol induction, Northern and Western blotting analyses indicated the expression of JEN1 in the transformants.
Lactate permease activity was obtained in P. Reconstitution of the lactate permease activity was achieved by fusing plasma membranes of P. These assays in reconstituted heterologous P. The highest V max 0. Thus overexpression of the S.
Jen1p is the only S. Functional expression of the lactate permease Jen1p of. In Sacchar omyces cer evisiae the activity for the lactate—proton. After 24 h of methanol induction, Northern and. W estern blotting analyses indicated the expression of JEN1. Lactate permease activity was obtained in.
Reconstitution of the lactate permease activity was achiev ed. These assays in reconstituted. Constitutive JEN1 expression and lactic acid uptake. Thus ov erexpression of the. J en1p is the only S. JEN1 , lactate transporter , Pichia pastoris , yeast. Lipophilic weak carboxylic acids are u sed as food preservativ es. The undissociated acid accumulates into microbial cells by simple. The acid dissociates in the cytoplasm,.